Optimization of Solvent Used for DNA Recovery of Gun Cartridges and Casings - A Proof of Concept Study
Abstract: Touch evidence, being one of the most common types of evidence found at a crime scene, offers the likelihood of having DNA. DNA recovery from this touch evidence is important and obtaining a high yield is optimal, but the current solvent used, water, does not provide high yields of DNA. Due to limited research being done, no optimal solvent has been determined for the use of DNA recovery in touch evidence (Van Oorschot, et al, 2010.) A proof of concept study was designed using an engineered fragment of DNA (plasmid DNA) to optimize a method for touch DNA recovery. Solvents such as ethanol, water, isopropanol, and sodium dodecyl sulfate (SDS) were tested to determine which solvent would provide a higher yield of plasmid DNA recovery from gun casings. The proof of concept involves the use of spectroscopy (Nanodrop) to compare the amount of DNA recovered from gun casing, to the amount originally placed on the casing. Next, recovered plasmid DNA was serially diluted and used as the template for a method that amplifies specific fragments of DNA. These fragments were visualized using gel electrophoresis. DNA isolated from touch evidence will be used for experimentation based upon results from the proof of concept study.
[s.l.] : [s.n.], 2610.
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